The four regional group champions play off over two legs for two of the promotion spots. Granada and Ponferradina won through, and Alcorcón and Sant Andreu go in to the second knock out stage. (23.05.10)
23 May | Alcorcón Iñigo López |
1-0 | Granada |
23 May | Ponferradina |
0-1 (9-8 pso) |
Sant Andreu Nacho (OG) |
The remaining twelve teams who finished in the top four of the regional groups entered a knock-out stage, with the winners of the six games entering a second round together with the two losers of the above two ties. Results were as follows (teams qualifying in bold).
22 May | Barcelona B Jonathan Soriano |
1-1 | Poli Ejido Antoñito |
23 May | Melilla |
0-3 | Universidad Las Palmas
Futre, Ángel (Pen), Vega |
23 May | Palencia Alejandro |
1-2 (aet) | Jaén Castellanos, Solabarrieta |
23 May | Oviedo Perona |
1-2 | Pontevedra Igor (2) |
23 May | Guadalajara Alain |
1-3 | Ontinyent Torres, Rubio, Collado |
23 May | Eibar Sutil (2, 1 Pen) |
2-0 | Alcoyano |
The four sides who finished fifth from bottom in the regional groups played-off to avoid the remaining two relegation spots. Espanyol B and Roquetas go down after losing their respective ties. (23.05.10)
22 May | Guijuelo Romero |
1-0 | Espanyol B |
23 May | Toledo Rufino |
1-1 | Roquetas Beltrán |